ANTIBIOFILM ACTIVITY AND STABILITY ASSAY OF PIGMENT FROM MARINE BACTERIA Bizionia paragorgiae

Abstract

Biofilms are communities that contain one or multiple microorganisms that enclosed in extracellular polymeric substance (EPS) matrix Biofilm formation is one of important survival skills for bacteria to be able to grow in all surfaces, such as living tissues, medical devices, industrial water piping, shipping, and natural aquatic system. It is recognized as the important virulence factor and protection for bacteria from environment influences. Bizionia paragorgiae, which produced orange pigment, was examined in this research. In this research, antibacterial, antibiofilm activity, and stability assay were examined. There was no antibacterial activity indicated from this study against all tested Gram-negative and Gram-positive bacteria. Based on antibiofilm activity against four pathogenic bacteria, both destruction and inhibition assay reduced biofilm formation by up to 30-70%. According to the result revealed from stability assay, the pigment was more stable at its natural pH 5.83, at 25 °C, and stored in dark condition.