BIODIVERSITY OF SPECIES AND ANTIMICROBIAL RESISTANCE OF BOVINE MILK WITH CLINICAL AND SUBCLINICAL MASTITIS

Abstract

This article was discussed the problems biodiversity of cow mastitis. The purpose of the work was to conduct a statistical analysis of microbiological data milk samples from cows with mastitis in order to ensure the targeted use of antibiotics on Ukrainian farms. Also, the article reveals the problems of the aseptic sampling technique for analysis, which complicates their identification. The obtained milk samples from 20 farm of Ukraine were examined using microbiology methods. As a result, 41% of all isolated bacteria were contagious mastitis agents: 15% of Staphylococcus aureus and 26% of Streptococcus agalactiae, and 59% were environmental mastitis agents. The most bacteria refer to Gram (+) microflora, namely streptococci (22.5% - Streptococcus spp. (S.agalactiae not include) and coagulase-negative stain (CNS) of staphylococci (14% - Staphylococcus spp. CNS). 18.5% of all isolated isolates are identified as Gram (-) microflora: for by Gram (-) microflora: 11% - E.coli, 6% -Pasteurella spp., 1.5% - Proteus spp. Mastitis caused by fungi (yeast), accounted for 1.5% of all diagnosed mastitis. Most of the isolates were sensitive to amoxicillin + clavulanic acid and gentamicin -93, 5%. The smallest number of isolates were sensitive to tylosin- 20,9% and streptomycin- 48,3%. A significant percentage (83.8% -54.8%) of obtained isolates were sensitive to rifampicin, amoxicillin, bacitracin, cloxacillin, trimethoprim, florfenicol, ampicillin, lincomycin, cephalexin, enrofloxacin, neomycin, penicillin.