ANTIBACTERIAL EFFECTS OF EXTRACTS FROM Tanacetum parthenium L. LEAVES

Authors

  • Zewditu Shiferaw
  • Sasikumar Jagathala Mahalingam Haramaya University
  • Ameha Kebede
  • Endale Teju

DOI:

https://doi.org/10.36547/be.394

Keywords:

bacterial pathogens, gram positive, gram negative, minimum inhibitory concentration, minimum bactericidal concentration, phytoconstituents, Tanacetum parthenium

Abstract

In our current study, phytochemical analysis and antibacterial activity of leaf extracts of T. parthenium were assessed. Qualitative phytochemical screening was carried using standard procedures and quantitative analysis for estimation of total phenolic content (TPC) was determined by spectrophotometric method. The antibacterial activity was accomplished using disc diffusion assay, determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against two gram positives (Streptococcus pyogenes (ATCC 19615), Streptococcus anginosus) and two gram negatives (Pseudomonas aeruginosa (ATCC 27853) and Fusobacterium necrogenes) pathogenic bacteria. Preliminary phytochemical analysis of the plant extracts revealed the presence of phenolics, tannins, flavonoids, terpenoids, and cardiac glycosides. The methanol extract had highest total phenolic content (6.17 ± 0.19 mg/ml). The results also displayed that the methanol extract exhibited significant antibacterial activity against the tested pathogenic microorganisms, in particular against S. anginosus with inhibition zone of 15.33±0.33 mm. The lowest MIC (33.33 mg/ml) and MBC (41.66 g/ml) values were observed in methanol extract against S. anginosus and S. pyogenes respectively. The results indicated that crude leaf extracts of  T. parthenium had a potent antibacterial activity suggesting that it could be valuable in treating pathogenic bacterial infections.

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Published

2022-01-28

How to Cite

Shiferaw , Z., Jagathala Mahalingam, S., Kebede, A. ., & Teju, E. . (2022). ANTIBACTERIAL EFFECTS OF EXTRACTS FROM Tanacetum parthenium L. LEAVES. Bacterial Empire, 5(1), e394. https://doi.org/10.36547/be.394

Issue

Section

Bacteriology Articles