ACTIVITY ASSAY OF SOME PROBIOTICS EXTRACT AS ANTI-Pseudomonas aeruginosa THAT ISOLATED FROM POSTOPERATIVE WOUND INFECTION IN ALNAJAF GOVRNORATE -IRAQ

Abstract

Background and objectives: Probiotics was redefined as “live microorganisms administered in inadequate amount that confer a health profit on the human for that the aim of this study were possibility of using probiotics extract with some supporting substances in antibacterial that resistant antibiotics.

Materials and methods:The methods of this study included isolated and identification of bacteria from postoperative wound infection of 50 women subjected to surgical birth process then selected the P.aeruginosa isolates and scanning the sensitivity test of some cephalosporin type of antibiotics and determine the presence of blaOXA-1 gene, also the study had tested activity of two types of probiotics (Bacillus clausii and Saccharomyces boulardii )extract with some supporting substances (acetic acid and citric acid) as antibacterial.

Results: The results of identification of bacterial isolates showed that 22(44%) isolates that related to Pseudomonas aeruginosa, 5(10%) isolates related to E.coli, 10(20%) isolates related to Klibsella spp., 10(20%) isolates related to Staphylococcus aureus and 3(6%) isolated related to Protus mirabils, and Overall, 22 isolates of P.aruginosa were analyzed by Kirby Bauer disk diffusion method and notice the resistance or sensitive to cephalexin, cefotriaxon and cefpime where the results confirmed 14 cephalosporin resistant isolates of Pseudomonas aeruginosa. Also detect blaOXA-1 gene by genotypic test. Amplification of b-lactamase genes shows the presence of blaOXA-1 (at 427 bp line) on among (10/22) study isolates. The results of antibacterial activity showed that inhibition zone of  probiotics extract only (14and 16)mm to S.bouldarii and B.clausii respectively but it showed synergism effect when supporting the S.bouldarii and B.clausii  extract with acetic acid where (18 and 20.3)mm respectively while (19 and 23.5)mm with citric acid respectively with significantly increasing differences when subjecting the bacteria to cefime ,ceftriaxone and cephlaxin antibiotics so,the inhibition zones were(1.3,10.3 and 0)mm respectively.

Conclusion :In light of the foregoing, we concluded the possibility of using probiotic extract with some supported substances as an anti-bacterial Pseudomonas aeruginosa isolated from surgical wound infection.