PRODUCTION OF LIPASE BY IMMOBILIZED BACILLUS THURINGIENSIS AND LYSINIBACILLUS SPHAERICUS AND THEIR BIODEGRADATION POTENTIAL ON DIESEL
Keywords:Bacillus thuringiensis, Lysinibacillus sphaericus, Immobilization, Biodegradation, Lipase
This study reported production of lipase by immobilized Bacillus thuringiensis. Bacteria isolates were screened on Bushnell-Hass Mineral Salt medium containing 1% v/v Diesel for oil degradation. The potent isolates were identified using 16S rRNA as Bacillus thuringiensis. The isolates were immobilized in gelatin matrix and cultured for lipase production in a submerged medium. The crude lipase extracted was used for degradation of Diesel. Optimum degradation of Diesel 41.4% was obtained by lipase from Immobilized Bacillus thuringiensis and 31.6% for Lysinibacillus sphaericus at pH 7 and 35 in 20 days. GC-MS analysis was carried out to show the compounds degraded after 20 days. This study therefore presented the use of immobilized bacterial lipase in degradation of Diesel as a simple and effective approach.
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