The ASSESSMENT OF THE ANTIBIOTIC RESISTANCE OF BACTERIAL ISOLATES RECOVERED FROM DOG FEED SOLD IN ADO-EKITI METROPOLIS

Abstract

Animal feed has been incriminated in many animal infectious diseases. This study was carried out to investigate and document the bacteriological safety of dog feed sold in Ado-Ekiti Metropolis. Five feed samples were analyzed, the bacterial load, coliform count, total Salmonella count as well as test for campylobacter was carried out on the feed samples. Pure culture of the isolates were subjected to antibiotic susceptibility test using disc diffusion method. The total bacterial count ranged between 4.41 log₁₀CFU/g and 6.36 log₁₀CFU/g. All feed samples had coliform ranging between 2.09 log₁₀CFU/g and 3.93 log₁₀CFU/g. Salmonella was only recovered from feed sample DED. Only sample DDL harbours Campylobacter sp. Other bacteria isolated from the feed were Escherichia coli, Bacillus sp, Enterobacter sp, Klebsiella sp, Staphylococcus sp and Lactobacillus sp. The antibiogram showed that nitofuratoin and ciproflocacin had the best activity where 93.10 % (54 out of 58) were susceptible to the two antibiotic.  Ampicilin was most resisted with only 36.21 % (21) susceptibility. Thirty one (31) of the isolates showed resistance to 3 or more antibiotic constituting 53.44 % of the entire bacterial isolate. Antibiotic resistance pattern mostly encountered were AMP, AMO, TLY and AMP, AMO, FUR with thirteen (13) isolates each. These results showed that dog feed may not be totally safe for the pets. Based on the type of bacteria isolated and the antibiotic resistance, good manufacturing practices should be ensured by manufactures in other to reduce the rate of contamination.