CHARACTERIZATION AND OPTIMIZATION OF KERATINOLYTIC BACTERIA Pseudomonas stutzeri C3 ISOLATED FROM POULTRY WASTE SOIL SAMPLE

Authors

  • Siddharthan Nagarajan Periyar University
  • Raja prabu Masilamni
  • Poongothai Eswaran
  • Kalaivani Govindaraj
  • Balagurunathan Ramasamy
  • Hemalatha Natarajan

DOI:

https://doi.org/10.36547/be.2019.2.4.103-108

Keywords:

Microbial degradation, keratinase, poultry farm, Pseudomonas stutzeri

Abstract

Microbial degradation of keratinous wastes is preferred over physicochemical methods, because it’s a cost effective and eco-friendly. Novel habitats are promising for discovery of new microbial strains. Towards isolate a keratinolytic bacteria, screening of bacterial strains from a poultry farm soil in Namakal district, Tamil Nadu, India was done and a promising the isolate C3 was found to degrade native chicken feather efficiently. It could grow over a pH range 7 at 40°C and in presence of potassium nitrate as a nitrogen source and citrate as a carbon source. Based on phenotypic characterization and 16S rRNA gene sequence analysis, the keratinolytic isolate was identified as Pseudomonas stutzeri C3. It produced 30.86±1.08 U/ml keratinase in raw feather medium and complete feather degradation at 5 days incubation periods.  

Downloads

Published

2019-12-05

How to Cite

Nagarajan, S., Masilamni, R. prabu, Eswaran, P., Govindaraj, K., Ramasamy, B., & Natarajan, H. (2019). CHARACTERIZATION AND OPTIMIZATION OF KERATINOLYTIC BACTERIA Pseudomonas stutzeri C3 ISOLATED FROM POULTRY WASTE SOIL SAMPLE. Bacterial Empire, 2(4), 103–108. https://doi.org/10.36547/be.2019.2.4.103-108

Issue

Vol. 2 No. 4 (2019): October November December

Section

Bacteriology Articles