COMPARATIVE METAGENOMIC PROFILING OF MICROBIAL COMMUNITIES IN HUMAN-IMPACTED AND PRISTINE STREAM WATER IN OLUGBADE VILLAGE, ISEYIN, OYO STATE

Abstract

Stream water, a major water source in rural communities, being impacted by human activities results to disturbance of the microbial ecosystem balance, pollution and impacts community health depending on such water. The study aimed to compare the metagenomic profiling of microbial communities in human-impacted and pristine stream water in Olugbade village, Oyo State, Nigeria. Human-impacted (sample A) and pristine (sample B) areas of the Stream were sampled following standard collection method. DNA extraction from the samples was performed using CTAB method and estimated through the Qubit ds DNA high sensitivity kit. Samples were sequenced on the Sequel system by PacBio (www.pacb.com). Raw subreads were processed through the SMRTlink (v9.0) Circular Consensus Sequences (CCS) algorithm to produce highly accurate reads (>QV40). These highly accurate reads were then processed through vsearch (https://github.com/torognes/vsearch) and taxonomic information was determined based on Qualitative Insights Into Microbial Ecology (QIMME2) version 2018.6.0. Results showed that organisms identified were 100% bacteria where sample A had 8,198 reads and B had 9, 827 reads. The taxonomic distribution revealed 23 and 43 phyla, 52 and 109 classes, 91 and 170 orders, 108 and 212 families, 211 and 336 genus and 277 and 455 species for sample A and B respectively. The study revealed that there is a statistical difference between human-impacted and pristine samples (p-value < 0.05). Sample B had more bacterial community than sample A thereby, showing that many of the bacterial naturally occurring in sample A are extinct or displaced due to different anthropogenic activities occurring there.