Hydrocarbon-Degrading Bacterial Strain Pseudomonas mendocina Newly Isolated from Marine Sediments and Seawater of Oran Harbor (Algerian Coast)

Authors

  • Bendadeche Medjahed Faiza Laboratory of aquaculture and Bioremediation, University of Oran 1 Ahmed ben Bella , Oran Algeria, Department of Biotechnology,

DOI:

https://doi.org/10.36547/ae.2020.2.2.22-29

Keywords:

Crude oil, Pseudomonas mendocina, Bioremediation, Marine sediments, 16S rDNA

Abstract

Contamination by petroleum hydrocarbons causes serious dangers to human health and the environment, whether by accidental or chronic contamination. Due to the large flow of ships, the commercial harbor of Oran is subject to pollution particularly by polycyclic aromatic hydrocarbons. For that, bioremediation by indigenous microorganisms is the most important method to eliminate or decrease this contamination. In the present paper, hydrocarbon-degrading bacterium strain SP57N has been studied, newly isolated from contaminated marine sediments and sea water from the harbor of Oran (Northwestern-Algeria), using of Bushnell-Hass salt medium (BHSM). The strain SP57N was Gram-negative, oxidase negative, catalase negative, motile, Rod-shaped bacteria, identified molecularly as Pseudomonas mendocina based on partial 16S rDNA gene sequence analysis, using the BLAST program on National Centre for Biotechnology Information (NCBI) and the EzBioCloud 16S rDNA databases. This isolate could growth on high concentrations of crude oil (up to 10 %, v/v). The effects of some culture conditions such as temperature, NaCl concentration and pH on growth rate of strain SP57N on crude oil as the sole carbon and energy source were studied. In addition, growth kinetic of this isolate on crude oil during 20 days of culture at 140 rpm, under optimal culture conditions was considered. The results showed maximum growth rate at temperature 25°C, 3% (w/v) of NaCl concentration and pH 7. Results of growth kinetic on crude oil as sole carbon and energy source showed that the stationary phase was attained at day 12. Thus, Pseudomonas mendocina SP57N had effectively hydrocarbon-degrading potential, and could be used as an efficacy degrader to initiate a biological eco-friendly method for the bioremediation of the hydrocarbon pollution on the port of Oran, and marine environment.

Published

2020-06-03

Issue

Section

Research Paper