Antifungal Activity of Topical Formulation Containing Artemisia Herba Alba Asso Essential Oil


  • Asma Boukhennoufa Laboratory of Bioconversion, Microbiology Engineering and Health Safety, Faculty SNV, University of Mascara, Algeria
  • Yamina Maizi
  • Aicha Tir Touil Meddah
  • Boumediene Meddah



Candida albicans, Essential oil, Artemisia herba alba Asso, Mascara, antifungal activity


Given the increase in antibiotic resistance phenomena and the undesirable effects of synthetic drugs. Medicinal plants are used as a direct or indirect source of the active ingredients. Our research constitutes a development of essential oils from Artemisia herba alba Asso cultivated in the Mascara region. A hydrophobic ointment has been formulated based on this essential oil and tested against five strains of Candida albicans. Several physicochemical and microbiological tests were used to verify the quality and the toxicity of the product. Then, the determination of the antifungal activity of this preparation was assessed against five strains of Candida albicans (S1, S2, S3, S4 and S5) by the disk-diffusion agar method. The results revealed that this preparation was devoid of total aerobic germs, yeasts and molds.  However, the pH value was found equal to 5.98.  In addition, the irritation primary index was marked less than 0.5. The ointment was powerful against S1, S3, S4 and S5 strains with inhibition diameters ranging from 16± 4 mm to 23 ± 2 mm. The Nystatin ointment was observed active against strains S2, S4 and S5 with diameters of the zones of inhibition; 21 ± 2 mm, 21± 1 mm and 20 ± 3 mm respectively. The ointment formulated with essential oil from Artemisia herba alba asso has proven useful against candidiasis caused by Candida albicans species.






Research Paper