The Isolation of BDCA-1 Myeloid Dendritic Cells from Bovine Blood for Detection of the Bovine Leukaemia Virus

Abstract

This article is focused on the isolation of CD1c+ dendritic cells (DCs) from bovine blood and their using for detection of bovine leukaemia virus. DCs are able to initiate an immune response because of that are antigen-presenting cells. DCs also play a key role in the regulation of innate immunity (NK cell function). Reciprocally, NK cells affect the activity of DCs. DCs have a few sub-groups. Myeloid DCs have two subsets – CD1c+ (BDCA-1) and CD141 (BDCA-3). We focused on detection of the glycoprotein gp51 in BDCA-1 cells infected by bovine leukaemia virus using immunofluorescence. We were able to detect bovine leukaemia virus in DCs which occurs within 8 years of age in cattle and it is the serious disease that needs to be detected in time.