POTENTIAL IN BIOETHANOL PRODUCTION FROM VARIOUS AGRO WASTES FERMENTING BY MICROORGANISMS USING CARROT PEEL, ONION PEEL, POTATO PEEL AND SUGAR BEET PEEL AS SUBSTRATES.
Keywords:Agro wastes, Bioethanol, Cladosporium cladosporioides, Saccharomyces cerevisiae, Gas Chromatography
Large amount of agro wastes are produced in Rwanda each year. The global annual potential bioethanol production from the major vegetables wastes such as carrot peel, onion peel, potato peel and sugar beet peel was estimated. Those wastes processing were successfully used as raw materials for the production of bioethanol, employing by cellulase produced from various filamentous fungi including Cladosporium cladosporioides was used for hydrolysis and the fermentation of the hydrolyzed samples was done using Sacharomyces cerevisiae. The fermented product was purified by primary distillation process at 79 °C and the fraction was collected. The ethanol is then determined by specific dichromate method and Gas Chromatography. Instantaneous saccharification and fermentation process yielded maximum ethanol in the substrate of carrot peel was 16.9 % at 21st day and further confirmed by Gas chromatography and the yield of ethanol obtained was 15.8 %.
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