TY - JOUR AU - Dindamo, Lire AU - Jagathala Mahalingam, Sasikumar AU - Getachew, Andualem AU - Kebede, Ameha PY - 2022/09/22 Y2 - 2024/03/29 TI - ANTIBACTERIAL ACTIVITIES OF ETHANOL EXTRACTS FROM Croton macrostachyus (Hochst. Ex Delile) AND Justicia schimperiana (Hochst. Ex Nees) BARK AND LEAF AGAINST CERTAIN PATHOGENIC BACTERIA JF - Bacterial Empire JA - BE VL - 5 IS - 4 SE - Bacteriology Articles DO - 10.36547/be.461 UR - https://office.scicell.org/index.php/BE/article/view/461 SP - e461 AB - <p>Our study was designed to evaluate antibacterial activities of ethanol extracts from <em>Croton macrostachyus</em> and <em>Justicia schimperiana</em> bark and leaf against certain bacterial pathogens such as <em>E. coli</em> O157 H:7 ATCC 25922, <em>Salmonella</em> Typhi ATCC 13311, <em>Staphylococcus aureus</em> ATCC 25923 and <em>Streptococcus pyogenes </em>ATCC 196151. Qualitative and quantitative methods were followed to screen the phytochemicals in the plants extracts and to estimate total phenolic content (TPC) and terpenoids. Antibacterial activity was evaluated by disc diffusion method and by determination of Minimum Inhibitory Concentration (MIC). The results of the investigation revealed that both the plants were found to contain alkaloids, saponin, steroids, tannins and terpenoids. Highest TPC (3.82±0.82 mg/g Tannic Acid Equivalent) and highest Terpenoid content (118.33±4.4 mg/g) were observed in the leaf extract of <em>C</em>. <em>macrostachyus</em>. Bark extract of <em>C</em>. <em>macrostachyus</em> displayed the strongest activity against the gram-positive bacteria <em>S. aureus</em> with an inhibition zone of 16.17±0.67 mm. <em>E. coli</em> O157 H:7 and <em>S</em>. Typhi were found more resistant to bark extract of <em>J. schimperiana </em>at the concentration of 100 mg/ml. The potent plant part extract should be subjected for further phytochemical analysis and bioactive investigations.</p> ER -