ANTIBACTERIAL EFFECTS OF EXTRACTS FROM Tanacetum parthenium L. LEAVES

Abstract

In our current study, phytochemical analysis and antibacterial activity of leaf extracts of T. parthenium were assessed. Qualitative phytochemical screening was carried using standard procedures and quantitative analysis for estimation of total phenolic content (TPC) was determined by spectrophotometric method. The antibacterial activity was accomplished using disc diffusion assay, determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against two gram positives (Streptococcus pyogenes (ATCC 19615), Streptococcus anginosus) and two gram negatives (Pseudomonas aeruginosa (ATCC 27853) and Fusobacterium necrogenes) pathogenic bacteria. Preliminary phytochemical analysis of the plant extracts revealed the presence of phenolics, tannins, flavonoids, terpenoids, and cardiac glycosides. The methanol extract had highest total phenolic content (6.17 ± 0.19 mg/ml). The results also displayed that the methanol extract exhibited significant antibacterial activity against the tested pathogenic microorganisms, in particular against S. anginosus with inhibition zone of 15.33±0.33 mm. The lowest MIC (33.33 mg/ml) and MBC (41.66 g/ml) values were observed in methanol extract against S. anginosus and S. pyogenes respectively. The results indicated that crude leaf extracts of  T. parthenium had a potent antibacterial activity suggesting that it could be valuable in treating pathogenic bacterial infections.